Installation of open source PyMOL|StructureDependencies
Python version: 3.12.x (https://www.python.org/downloads/release/python-3120/)
PyMOL whl: https://github.com/cnpem/PyMOL4Win/tree/main/wh ...
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Single cell curated markers|Omics|Single cellHere's something encrypted, password is required to continue reading.
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ggplot2绘图参数|R图例
删除图例: theme(legend.position = "none")
删除图例标题: theme(legend.title = element_blank());guides(color = guide_legend(title = NULL))
修改图例标题: la ...
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下载EGA测序数据|Omics下载EGA数据
创建python 3.6环境:conda create -n EGA python=3.6
python3.6才能正常运行
安装pyEGA3:pip3 install pyega3
pyega3: https://github.com/EGA-archive/ega-do ...
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上传与下载NCBI测序数据|Omics上传数据到NCBI
进入上传网址: https://submit.ncbi.nlm.nih.gov/
选择上传的数据库
Sequence Read Archive (SRA): 上传测序数据
GEO: 转录组或表观组数据(上传表达矩阵和测序数据)
填写项目和样本信息
上传数据
通过Fil ...
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Linux部署Jupyter|Linux安装Jupyter和IPythonpip install ipythonpip install jupyter
IPthon生成密码ipython
12from jupyter_server.auth import passwdpasswd()
配置Jupyterjupyter notebook ...
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Perl安装模块|OtherPerl安装模块
模块下载地址: https://metacpan.org/
安装: perl Makefile.PL make make install
当出现问题Can’t locate xxx.pm in @INC时安装模块可以解决
交互安装模块: perl -MCPAN -e shell i ...
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CUTTag Pipeline|Omics|EpigenomeReferences: https://yezhengstat.github.io/CUTTag_tutorial/
Alignment–Bowtie21234567891011121314151617# Build hg38 indexbowtie2-build GRCh38.p14.genome ...
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ChIP-Seq Pipeline|Omics|EpigenomeReferences:
http://biocluster.ucr.edu/~rkaundal/workshops/R_feb2016/ChIPseq/ChIPseq.html
https://github.com/ENCODE-DCC/chip-seq-pipeline2
Quality C ...
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DESeq2差异表达分析|Omics|Transcriptometximport导入表达定量文件获取转录本ID所对应的基因Id1234library(GenomicFeatures)txd <- makeTxDbFromGFF(file='gencode.v44.annotation.gtf',format='gtf' ...
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