Installation of open source PyMOL
Dependencies Python version: 3.12.x (https://www.python.org/downloads/release/python-3120/) PyMOL whl: https://github.com/cnpem/PyMOL4Win/tree/main/wh ...
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Single cell curated markers
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ggplot2绘图参数
图例 删除图例: theme(legend.position = "none") 删除图例标题: theme(legend.title = element_blank());guides(color = guide_legend(title = NULL)) 修改图例标题: la ...
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下载EGA测序数据
下载EGA数据 创建python 3.6环境:conda create -n EGA python=3.6 python3.6才能正常运行 安装pyEGA3:pip3 install pyega3 pyega3: https://github.com/EGA-archive/ega-do ...
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上传与下载NCBI测序数据
上传数据到NCBI 进入上传网址: https://submit.ncbi.nlm.nih.gov/ 选择上传的数据库 Sequence Read Archive (SRA): 上传测序数据 GEO: 转录组或表观组数据(上传表达矩阵和测序数据) 填写项目和样本信息 上传数据 通过Fil ...
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Linux部署Jupyter
安装Jupyter和IPythonpip install ipythonpip install jupyter IPthon生成密码ipython 12from jupyter_server.auth import passwdpasswd() 配置Jupyterjupyter notebook ...
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Perl安装模块
Perl安装模块 模块下载地址: https://metacpan.org/ 安装: perl Makefile.PL make make install 当出现问题Can’t locate xxx.pm in @INC时安装模块可以解决 交互安装模块: perl -MCPAN -e shell i ...
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CUTTag Pipeline
References: https://yezhengstat.github.io/CUTTag_tutorial/ Alignment–Bowtie21234567891011121314151617# Build hg38 indexbowtie2-build GRCh38.p14.genome ...
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ChIP-Seq Pipeline
References: http://biocluster.ucr.edu/~rkaundal/workshops/R_feb2016/ChIPseq/ChIPseq.html https://github.com/ENCODE-DCC/chip-seq-pipeline2 Quality C ...
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DESeq2差异表达分析
tximport导入表达定量文件获取转录本ID所对应的基因Id1234library(GenomicFeatures)txd <- makeTxDbFromGFF(file='gencode.v44.annotation.gtf',format='gtf' ...
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