测序数据生成表达矩阵

Cell Ranger count

# Fastq name must be: sample_S1_L00[lane_number]_[I1/R1/R2]_001.fastq.gz
# I1 is optional
cellranger count \
--sample=sampleName \
--fastqs=/opt/data \
--id=out \
--transcriptome=/opt/cellranger/refdata-gex-GRCh38-2020-A   

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Cell Ranger和参考基因组下载: https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest (填写相关信息下载, Cell Ranger解压就可以用)