Load expression data123456789library(Seurat)library(ggplot2)# Read count data (row is gene, column is cell)data <- read.table(file = "sample.c ...

测序数据生成表达矩阵Cell Ranger count1234567# Fastq name must be: sample_S1_L00[lane_number]_[I1/R1/R2]_001.fastq.gz# I1 is optionalcellranger count \--sample=s ...

测序数据生成表达矩阵质控Fastp1234567fastp -i sample.raw.r1.fq.gz \-I sample.raw.r2.fq.gz \-o sample.clean.r1.fq.gz \-O sample.clean.r2.fq.gz \-j sample.QC.json \- ...

以下均以人Hg38(GRCh38)版本为例 Ensembl https://asia.ensembl.org/Homo_sapiens/Info/Index Fasta: Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz (https://ftp.ense ...

提取文件提取fasta文件samtools faidx genome.fa chr1 > genome_chr1.fasamtools faidx genome.fa chr1:1-1000 > genome_chr1.fa 提取bam文件samtools view -hb sample ...

R version: 4.2.1 Seuratinstall.packages("Seurat") rgeos包依赖geos-config: 下载geos: http://download.osgeo.org/geos/geos-3.9.3.tar.bz2 安装geos: ...

R 4.2.1安装 R下载地址: https://mirrors.ustc.edu.cn/CRAN/ 安装依赖:1234567891011121314yum install libcurl-develyum install readline-develyum install libXt-devely ...

Conda安装 Conda下载地址: https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/ Conda安装: bash Miniconda3-latest-Linux-x86_64.sh Conda添加仓库conda config --a ...

Modules基本命令 显示可以使用的模块: module avail/ava 加载模块: module load/add R/4.1.3 卸载模块: module unload/rm R/4.1.3 显示已加载的模块: module list 卸载所有加载模块: module purge 查看模块 ...

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